Comparison of on-capillary derivatization of amino acids using different strategies of reactant mixing for capillary electrophoresis coupled with LIF
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| Year of publication | 2016 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Amino acids are very important compounds for living organisms. They exhibit low or no native fluorescence, thus derivatization is essential for their sensitive determination by laser induced fluorescence detection. Derivatization reaction is often time consuming, laborious and derivatization dyes are quite expensive. Capillary electrophoresis offers conducting the derivatization reaction in pre-capillary, on capillary or post capillary arrangement. In the case of on-capillary derivatization, mixing of the reactants can be performed by longitudinal diffusion, by transverse diffusion of laminar flow profiles (TDLFP), or by electrophoretically mediated microanalysis (EMMA). Two methods for on-capillary derivatization of all standard of amino acids with naphthalene 2,3 dicarboxaldehyde/sodium cyanide were optimized using mixing of reactants both by TDLFP and EMMA and compared in terms of efficiency of mixing of reactants, sensitivity, repeatability and applicability to various biological samples. |
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