Multiple hypernation of LC with ESI MS, MALDI MS and SALD ICP MS - the detection platform for metalloproteomic studies

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Authors

COUFALÍKOVÁ Kateřina BENEŠOVÁ Iva DLABKOVÁ Kristýna KANICKÝ Viktor PREISLER Jan

Year of publication 2016
Type Article in Proceedings
Conference CECE 2016 13th International Interdisciplinary meeting on bioanalysis
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords MALDI MS; ESI MS; SALD ICP MS; metallothioneins
Description We present a new methodology for coupling conventional on-line LC-ESI MS with two off-line detection techniques: matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) analyses. The Off-line approach is facilitated using a commercial MALDI target made of conductive plastics, which is suitable for the both MALDI MS and SALD ICP MS. Due to this arrangement, two types of analysis, molecular and elemental determination of metallothionein 1 (MT-1) were performed.
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