Environmental Scanning Electron Microscopy as a Useful Tool for Taxonomical Documentation of Parasitical Helminths

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Authors

TIHLAŘÍKOVÁ Eva NEDĚLA Vilém MAŠOVÁ Šárka

Year of publication 2016
Type Article in Periodical
Magazine / Source Microscopy and Microanalysis
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1017/S1431927616006735
Field Zoology
Keywords Helminthiases; Parasites; ESEM
Description The most commonly used method for parasitological studies is scanning electron microscopy (SEM). Sometimes conventional SEM is not acceptable because only a small number or only one sample of a rare parasite is available. The purpose of this work is to show the results of unique observations of basics groups of helminth parasites (roundworms, tapeworms, flukes and spiny headed worms) by environmental scanning electron microscopy (ESEM) with minimal shape and volume deformations. We also show the advantages of studying of these specific types of biological samples in controlled dynamically changing conditions in the specimen chamber of a non-commercial ESEM AQUASEM II.
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