SDS-PAGE and Gel IEF: Tool for Differentiation of Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus aureus

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Authors

TESAŘOVÁ Marie HORKÁ Marie MORAVCOVÁ Dana SVOJANOVSKÁ Lenka MLYNÁRIKOVÁ Katarína RŮŽIČKA Filip

Year of publication 2016
Type Article in Periodical
Magazine / Source Current Microbiology
MU Faculty or unit

Faculty of Medicine

Citation
Web http://link.springer.com/article/10.1007%2Fs00284-015-0939-y
Doi http://dx.doi.org/10.1007/s00284-015-0939-y
Field Microbiology, virology
Keywords LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; CAPILLARY; ELECTROPHORESIS; BACTERIA; PROTEINS
Description The methicillin-resistant Staphylococcus aureus causes difficult-to-treat healthcare-associated infections in humans. For fast and effective selection of an appropriate antibiotic therapy, it is essential to have rapid and reliable methods for differentiation of methicillin-resistant S. aureus from less dangerous methicillin-sensitive S. aureus. There have been many methods for the identification of methicillin-resistant S. aureus described but none has been accepted as an international standard. The most commonly used techniques such as phenotyping and genotyping have a few disadvantages, for instance, these techniques are not reproducible and stable. In addition, they are time-consuming, expensive, and they are not capable to distinguish all S. aureus strains. In this study, the methicillin-resistant and methicillin-sensitive S. aureus isolates obtained from patients were extracted in hot water. The released proteins were characterised by sodium dodecyl sulphate polyacrylamide gel electrophoresis and gel isoelectric focusing. These two methods were able to differentiate among tested bacterial strains. The proposed methods are time saving, they are applicable in standard biochemical laboratories, and they do not require any expensive equipment.
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