Imunologické aspekty v onkologii - cirkulující gamma delta T lymfocyty
| Title in English | Immunological Aspects in Oncology - Circulating gamma delta T Cells |
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| Authors | |
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Klinická onkologie |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.14735/amko20152S60 |
| Field | Oncology and hematology |
| Keywords | gamma delta T cells; cancer; immune system; peripheral blood; immunotherapy; T lymfocytes |
| Description | Gamma delta T cells present a minor population of the cell family which basically differs in construction of their T cell receptor (TCR), Thanks to the features of gamma delta TCR, these cells can acquire unique effector functions and play a specific role (not only) in anti-tumor immune response. In this article, we describe the basic characteristics of this cell population and their connection to cancer. In the experimental part we performed exploratory analysis of circulating gamma delta T cells in reference population and comparison with melanoma and breast carcinoma patients. The median percentage of gamma delta T cells from all lymphocytes was 2.9% (interquartile range-IQR 1.7-4%), The median absolute numbers of y6 cells per liter of blood was 5.05 x 107 (IQR 2.9-7.84 x 107). The median percentage of y6 cells between all CD3 T cells was 3.9% (IQR 2.3-5.6%). No correlation between gamma delta T cells levels and gender or age was observed in reference population. Detailed immunophenotyping was also conducted describing representation of memory subsets (using CD45RO and CD27 markers) and presence of surface markers HLA-Dr, CD69, CD25, CD28, CCR7, CTLA-4, ICOS, PD-1 Land PD-1 between gamma delta T cells of the controls and breast carcinoma patients. From this analysis, it is evident that gamma delta T cells do not represent a uniform population but they differ in surface markers as well as in their effector functions. |
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