HotSpot Wizard 2.0

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Authors

ŠTOURAČ Jan BENDL Jaroslav ŠEBESTOVÁ Eva VÁVRA Ondřej MUSIL Miloš BREZOVSKÝ Jan DAMBORSKÝ Jiří

Year of publication 2015
MU Faculty or unit

Faculty of Science

Web http://loschmidt.chemi.muni.cz/hotspotwizard2/
Description HotSpot Wizard is a web server for automatic identification of hot spots for engineering of substrate specificity, activity or entantioselectivity of enzymes and for annotation of protein structures. HSW 2.0 integrates 3 databases and 18 computational tools, including our in-house tool for analysis of protein tunnels and channels Caver 3.0. In the output, users can explore hot spots based on 4 protein engineering strategies: (i) functional hot spots represented by highly mutable residues located in the active site pocket or access tunnels, (ii) stability hot spots represented by flexible residues, (iii) stability hot spots identified by back-to-consensus approach, and (iv) correlated hot spots represented by pairs of coevolving residues that modulate enzyme activity and selectivity.
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