HotSpot Wizard 2.0

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Authors	ŠTOURAČ Jan BENDL Jaroslav ŠEBESTOVÁ Eva VÁVRA Ondřej MUSIL Miloš BREZOVSKÝ Jan DAMBORSKÝ Jiří
Year of publication	2015
MU Faculty or unit	Faculty of Science
Web	http://loschmidt.chemi.muni.cz/hotspotwizard2/
Description	HotSpot Wizard is a web server for automatic identification of hot spots for engineering of substrate specificity, activity or entantioselectivity of enzymes and for annotation of protein structures. HSW 2.0 integrates 3 databases and 18 computational tools, including our in-house tool for analysis of protein tunnels and channels Caver 3.0. In the output, users can explore hot spots based on 4 protein engineering strategies: (i) functional hot spots represented by highly mutable residues located in the active site pocket or access tunnels, (ii) stability hot spots represented by flexible residues, (iii) stability hot spots identified by back-to-consensus approach, and (iv) correlated hot spots represented by pairs of coevolving residues that modulate enzyme activity and selectivity.
Related projects:	Biosenzor pro monitorování toxických látek v životním prostředí