Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells

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Authors

KLISZCZAK Maciej SEDLÁČKOVÁ Hana PITCHAI Ganesha P. STREICHER Werner W. KREJČÍ Lumír HICKSON Ian D.

Year of publication 2015
Type Article in Periodical
Magazine / Source Oncotarget
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.18632/oncotarget.6342
Field Genetics and molecular biology
Keywords DNA replication; RecQ helicases; minichromosome maintenance proteins; Chromosome Section
Description DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.
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