Retention behavior of trinucleotides in electrostatic repulsion hydrophilic interaction chromatography

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Authors

FIALA Jan BITTOVÁ Miroslava HAVLIŠ Jan

Year of publication 2015
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) was firstly introduced by Andrew J. Alpert in 2008 as an alternative chromatographic mode for separation of highly charged solutes such as peptides, amino acids and nucleotides. Combination of hydrophilic interactions and electrostatic attraction/repulsion in mentioned mixed mode chromatography offers efficient isocratic elution of solutes which would normally require gradient elution for their separation. Nowadays, the ERLIC mode is utilized mostly in proteomic research, nevertheless, its advantages call for the application in other biological fields, e.g. in polynucleotide analysis.
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