Retention behavior of trinucleotides in electrostatic repulsion hydrophilic interaction chromatography

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Authors	FIALA Jan BITTOVÁ Miroslava HAVLIŠ Jan
Year of publication	2015
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) was firstly introduced by Andrew J. Alpert in 2008 as an alternative chromatographic mode for separation of highly charged solutes such as peptides, amino acids and nucleotides. Combination of hydrophilic interactions and electrostatic attraction/repulsion in mentioned mixed mode chromatography offers efficient isocratic elution of solutes which would normally require gradient elution for their separation. Nowadays, the ERLIC mode is utilized mostly in proteomic research, nevertheless, its advantages call for the application in other biological fields, e.g. in polynucleotide analysis.
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