Determination of biochemically important flavins using capillary electrophoresis with laser induced fluorescence detection
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| Year of publication | 2015 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Riboflavin, known as vitamin B2, is a precursor of important redox cofactors - flavin mononucleotide and flavin adenine dinucleotide. Its basic structure contains isoalloxazine consisting of three condensed heterocycles. Rigidity of this structure is responsible for native fluorescence of flavins and therefore can be easily and sensitively detected using fluorescence detection without derivatization. Optimization of separation method based on capillary electrophoresis coupled with laser induced fluorescence detection for determination of riboflavin in urine and beverages is subject of this contribution. |
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