N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications

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Authors	GOLUNOVA A. JAROŠ Josef JURTÍKOVÁ Veronika KOTELNIKOV I. KOTEK J. HLÍDKOVÁ H. STREIT Libor HAMPL Aleš RYPÁČEK F. PROKS V.
Year of publication	2015
Type	Article in Periodical
Magazine / Source	Physiological Research
MU Faculty or unit	Faculty of Medicine
Citation
Field	Genetics and molecular biology
Keywords	Hydrogels; Cryogels; Stem cells; Scaffolds; Mechanical properties
Description	The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.
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