Potenciální účinky léčiv v akvatickém prostředí
| Title in English | Potential effects of pharmaceuticals in aquatic environment |
|---|---|
| Authors | |
| Year of publication | 2015 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Pharmaceuticals represent a group of water contaminants with increasing importance. Residues of their active substances or products of their transformation can affect even non-target organisms. Studies demonstrating their effect to plants are, in comparison to studies on microorganisms and animals, scarce. Higher aquatic plant Lemna minor is often used in phytotoxicity tests for evaluation the risk of various xenobiotics (US EPA, OECD). The aim of this study was to evaluate the growth, biochemical and histochemical changes in duckweed exposed for 10 days to non-sterodial anti-inflammatory drugs diclofenac (DCF) and paracetamol (PCT)(0.1, 10 and 100 µg/L). Despite the high content of both drugs in duckweed plants, only 100µg/L DCF inhibited the biomass production. To the contrary, lower concentrations exhibited significant changes at biochemical and histochemical level: decrease of photosynthetic pigments content and values of induced chlorophyll fluorescence parameters (FV/FM, Rfd), increased production of reactive oxygen (ROS) and nitrogen (RNS) and subsequent increased aktivity of antioxidative defense systems (higher content of oxidized thiols, activity of glutathione-reductase), or decrease in membrane function and lowered cell viability. Evaluation of biochemical and histochemical markers is earlier and more sensitive indication of the effect of micropollutants like drugs than usually assessed growth parameters. |