Determination of binding constant by capillary electrophoresis - frontal analysis and isothermal titration calorimetry
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| Year of publication | 2015 |
| Type | Conference abstract |
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| Description | In this study, the binding constant was determined by two methods: capillary electrophoresis – frontal analysis (CE FA) and isothermal titration calorimetry (ITC). Both CE FA and ITC measure the affinity of binding partners in their native states without immobilization or labelling of the interacting partners. What is more we can simulated physiological conditions because the investigated interactions takes place in a solution. Disadvantages of ITC are a large sample consumption and a problem with estimation of week affinity interactions [2], the advantage of CE-FA are a very low sample consumption and a high resolution and also it does not require the highly purified samples. |
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