Use of flower-like gold nanoparticles in time-of-flight mass spectrometry

Investor logo

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

KOLÁŘOVÁ Lenka KUČERA Lukáš VAŇHARA Petr HAMPL Aleš HAVEL Josef

Year of publication 2015
Type Article in Periodical
Magazine / Source Rapid Communication in Mass Spectrometry
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1002/rcm.7265
Field Analytic chemistry
Keywords Flower-like gold nanoparticles; MALDI; SALDI; TOF mass spectrometry; biomolecules
Description Many kinds of nanoparticles (NPs) have been used for mass spectrometry (MS) so far. Here we report the first use of flower-like gold nanoparticles (AuNPs) as a mediator enhancing ionization in MS of peptides and proteins. Flower-like AuNPs were characterized using transmission and scanning electron microscopy, UV-VIS spectrophotometry, and laser desorption/ionization (LDI) MS and compared with polyhedral AuNPs. Mass spectra were obtained in positive ion mode using time-of-flight (TOF) analyzer coupled with either matrix assisted laser desorption/ionization (MALDI) or surface assisted laser desorption/ionization (SALDI) methods. The intensities of peptide peaks (m/z 500–3500) were up to 7.5× and up to 7× higher using flower-like AuNPs and flower-like AuNPs-enriched cyano-4-hydroxycinnamic acid (CHCA) matrix respectively, than the classical CHCA matrix. The signals of higher mass peptide/protein peaks (m/z 3600–17000) were up to 2× higher with using flower-like AuNPs enriched CHCA matrix than conventional CHCA matrix. The signal of profile peaks generated by intact cell MALDI-TOFMS of fibroblast suspension (m/z 4000–20000) was 2× higher with using flower-like AuNPs combined with sinapinic acid (SA) compared to SA matrix alone. The use of flower-like AuNPs as internal calibration standard for the calibration of MS spectra of peptides was performed. Flower-like AuNPs and flower-like AuNPs combined with CHCA or SA as combined matrices for MS measurement of peptides and proteins were used. Comparison of conventional MALDI method and our method with flower-like AuNPs was carried out. In addition, gold clusters generated from flower-like AuNPs by SALDI provide suitable internal calibration standard for MS analysis of peptides.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info