Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

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Authors	SUN Xiaolong LACINA Karel RAMSAMY Elena C. FLOWER Stephen E. FOSSEY John S. QIAN Xuhong ANSLYN Eric V. BULL Steven D. JAMES Tony D.
Year of publication	2015
Type	Article in Periodical
Magazine / Source	CHEMICAL SCIENCE
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://pubs.rsc.org/en/content/articlelanding/2015/sc/c4sc03983a#!divAbstract
Doi	http://dx.doi.org/10.1039/c4sc03983a
Field	Analytic chemistry
Keywords	APPENDED BIPYRIDINIUM SALTS; BORONIC ACID; HYDROGEN-PEROXIDE; NITRIC-OXIDE; FLUORESCENT-PROBES; SENSING SYSTEM; GLUCOSE; CHEMOSENSOR; REPORTER; VIOLOGEN
Description	Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS–NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B–N interaction. Our simple system produces a visible colorimetric change and on–off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reactionbased Indicator displacement Assay).
Related projects:	CEITEC - Central European Institute of Technology