Role of c-Myb in chondrogenesis
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Bone |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1016/j.bone.2015.02.031 |
| Field | Genetics and molecular biology |
| Keywords | micromass cultures; mouse limbs; endochondral bone; c-Myb; chondrogenesis; |
| Description | To investigate the role of c-Myb in endochondral ossification, we carried out our study on the long bones of mouse limbs during embryonic development. First, the c-myb expression pattern was analyzed by in situ hybridization during endochondral ossification of long bones. The c-Myb protein was found in proliferating chondrocytes and in the perichondrium of the forelimb bones (E14.5–E17.5). Furthermore, protein was detected in pre-hypertrophic as well as hypertrophic chondrocytes. Gain-of-function and loss-of-function approaches were used to test the effect of altered c-myb expression on chondrogenesis in micromass cultures established from forelimb buds of mouse embryos. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulated the level of Sox9 expression, a major marker of chondrogenesis. Transient c-myb overexpression markedly increased the formation of cartilage nodules and the production of extracellular matrix as detected by intense staining with Alcian blue. Taken together, the results of this study demonstrate that the c-Myb transcription factor is involved in the regulation and promotion of endochondral bone formation. |
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