Analýza malých změn Fc-glykosylace IgG s využitím izotopového značení glykopeptidů

• Title in English: Analysis of IgG Fc-glycosylation using glycopeptide stable isotope labeling
• Authors: PABST Martin; BENEŠOVÁ Iva; FAGERER Stephan Rupert; JACOBSEN Mathias; EYER Klaus; SCHMIDT G.; KRISMER Jasmin; WAHL F.; PREISLER Jan; ZENOBI Renato
• Year of publication: 2015
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: For the first time, stable isotope labeling of the glycopeptide was used for IgG N-glycosylation analysis. Succinic anhydride was found as a suitable label as it can be prepared in natural (light) and heavy (D413C4) form. A sample preparation protocol consisting of an in-solution tryptic digestion, a „one-pot“ labeling and a SPE was developed. Labeled glycopeptides were analyzed using MALDI MS and LC-ESI MS.

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