Analýza malých změn Fc-glykosylace IgG s využitím izotopového značení glykopeptidů
| Title in English | Analysis of IgG Fc-glycosylation using glycopeptide stable isotope labeling |
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| Authors | |
| Year of publication | 2015 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | PABST, Martin, Iva BENEŠOVÁ, Stephan Rupert FAGERER, Mathias JACOBSEN, Klaus EYER, G. SCHMIDT, Jasmin KRISMER, F. WAHL, Jan PREISLER and Renato ZENOBI. Analýza malých změn Fc-glykosylace IgG s využitím izotopového značení glykopeptidů (Analysis of IgG Fc-glycosylation using glycopeptide stable isotope labeling). In 4. Konference České společnosti pro hmotnostní spektrometrii. 2015. ISBN 978-80-905045-4-7. |
| Description | For the first time, stable isotope labeling of the glycopeptide was used for IgG N-glycosylation analysis. Succinic anhydride was found as a suitable label as it can be prepared in natural (light) and heavy (D413C4) form. A sample preparation protocol consisting of an in-solution tryptic digestion, a „one-pot“ labeling and a SPE was developed. Labeled glycopeptides were analyzed using MALDI MS and LC-ESI MS. |
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