Determination of serum total protein by the Kjeldahl method

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Authors

VINKLÁRKOVÁ Bára CHROMÝ Vratislav BITTOVÁ Miroslava ŽALUDOVÁ Lenka

Year of publication 2015
Type Article in Proceedings
Conference Študentská vedecká konferencia PriF UK 2015
MU Faculty or unit

Faculty of Science

Citation
Web http://2015.svkprifuk.info/zbornik2015.pdf
Field Analytic chemistry
Keywords total protein; kjeldahl method; biuret method
Description Chromý et al. found in 2009, that albumin-calibrated analysis of serum total protein according to a candidate reference method, developed by Doumas working group in 1981, provide results with unacceptable positive bias. The simplest way to rectify this defect is the use of serum and/or plasma-based protein standards certified by the Kjeldahl method. We reviewed Kjeldahl methods adopted by laboratory medicine and selected for total protein a direct analysis on washed protein precipitates with trichloroacetic acid and verified its preliminary analytical performance parameters. We describe a final simplified analysis with modified still unit and digestion flask serving for both digestion and distillation and present its re-validation.
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