Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Chemical Reviews |
| MU Faculty or unit | |
| Citation | |
| web | http://pubs.acs.org/doi/pdf/10.1021/cr500279h |
| Doi | http://dx.doi.org/10.1021/cr500279h |
| Field | Electrochemistry |
| Keywords | Electrochemistry; Proteins; Glycoproteins; Sensors |
| Description | In this review, we wish to show that in the recent years a significant progress was done in the EC analysis of practically all proteins, based on electroactivity of amino acid (aa) residues in proteins. Also electrochemistry of polysaccharides, oligosaccharides and glycoproteins greatly advanced in creating important steps for its larger application in the glycoprotein research. In recent decades, a great effort was devoted to the discovery and application of biomarkers for analysis of different diseases, including cancer. In the following paragraphs, special attention will be paid (i) to intrinsic electroactivity of peptides and proteins, including the sensitivity to changes in protein 3D structures, as well as to recent advances in EC investigations of DNA-protein interactions, (ii) to intrinsic electroactivity of glycans and polysaccharides, advances in EC detection of lectin-glycoprotein interactions and to introduction of electroactive labels to polysaccharides and glycans and finally (iii) to EC detection of protein biomarkers, based predominantly on application of antibodies in immunoassays, nucleic acid and peptide aptamers for construction of aptasensors, and lectin biosensors for detection of glycoprotein biomarkers. |
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