Detekce genově specifických mikrosatelitních markerů jetele lučního
| Title in English | Identification of gene specific microsatellite markers in red clover |
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| Authors | |
| Year of publication | 2014 |
| MU Faculty or unit | |
| Description | Method and procedure concern set of microsatellite markers in specific genes of Trifolium pratense (red clover) based on our own next generation sequencing. The set includs species specific SSR markers in coding sequences (exons) for desaturases, polyphenol oxidases and nodulins participating in biosynthesis of fatty acids, proteine quality and nitrogen fixation, in addition of markers in genes for resistance and yield. Methods includ specific oligonucleotides and amplification cycles of targeted SSR markes. The marker set has been intended for red clover breeding. |
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