Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water

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Authors

HORKÁ Marie KARÁSEK Pavel RŮŽIČKA Filip DVOŘÁČKOVÁ Milada SITTOVÁ Martina ROTH Michal

Year of publication 2014
Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Faculty of Medicine

Citation
Web http://pubs.acs.org/doi/abs/10.1021/ac502254f
Doi http://dx.doi.org/10.1021/ac502254f
Field Analytic chemistry
Keywords FLIGHT MASS-SPECTROMETRY; BACTERIAL ADHESION; ZONE-ELECTROPHORESIS; ISOELECTRIC POINT; CHROMOGENIC AGAR; SPECTRA MRSA; UV DETECTION; MICROORGANISMS; STRAINS; IDENTIFICATION
Description Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fasgt and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supecritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3 glycidyloxypropyl)trimethoxysilane. With optimized proportions fo suitable additives to the background electrolyte, a CZE separation of MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. the isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI= 3.4.
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