Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | 11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014 |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | capillary electrophoresis; fluorescence detection; derivatization; amino acids |
| Description | Amino acids are very important compounds in living organisms and their quantification in biological fluids can be used for diagnostic purposes. For determination of amino acids by capillary electrophoresis coupled with laser induced fluorescence detection, derivatization procedure is necessary because only a few amino acids exhibit native fluorescence. Derivatization reagent naphthalene-2,3-dicarboxaldehyde reacts with primary amines in the presence of nucleophile, such as cyanide anion, to form fluorescent derivates. Derivatization reaction can be done in pre-capillary or on-capillary arrangement. In on-capillary arrangement the capillary is used both for separation and derivatization. Optimization of pre-capillary and on-capillary derivatization procedure of amino acids using naphthalene-2,3-dicarboxaldehyde and NaCN, and comparison of these approaches is subject of this contribution. |
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