Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods

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Authors

ŠIMARA Pavel TESAŘOVÁ Lenka PAĎOUROVÁ Stanislava KRONTORÁD KOUTNÁ Irena

Year of publication 2014
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Informatics

Citation
Description Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfill this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during 7 years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSCs clones are highly preferable in terms of future clinical application. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human fibroblasts and CD34+ blood progenitors were used as a source cells and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions.
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