New sensitive detection method for lectin hemagglutination using microscopy.
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Periodical |
| Magazine / Source | Microscopy research and technique |
| MU Faculty or unit | |
| Citation | |
| Web | http://dx.doi.org/10.1002/jemt.22407 |
| Doi | http://dx.doi.org/10.1002/jemt.22407 |
| Field | Biochemistry |
| Keywords | hemagglutination; protein-carbohydrate interactions; blood group antigens; lectin |
| Description | The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope. Comparison with the standard hemagglutination assay using microtiter plates revealed that microscopic approach is faster and more robust and allows fast determination of lectin activities immediately in bacterial cytosols. |
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