Voltammetric Study of dsDNA Modified by Multi-redox LabelBased on N-methyl-4-hydrazino-7-nitrobenzofurazan

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Authors

DAŇHEL Aleš RAINDLOVA Veronika HAVRAN Luděk BAREK Jiří HOCEK Michal FOJTA Miroslav

Year of publication 2014
Type Article in Periodical
Magazine / Source Electrochimica Acta
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.sciencedirect.com/science/article/pii/S001346861400468X#
Doi http://dx.doi.org/10.1016/j.electacta.2014.02.137
Field Electrochemistry
Keywords DNA; Redox labeling; N-methyl-4-hydrazino-7-nitrobenzofurazan; Silver solid amalgam electrode; Voltammetry
Description Voltammetric behaviour of novel synthetic multi-redox DNA label N-methyl-4-hydrazino-7-nitrobenzofurazan (NBF), a deoxycytidine monophosphate conjugate with NBF via formylthiophenelinker (dCNBFMP), and NBF labeled model short double stranded DNA (dsDNA-NBF) were systematicallystudied at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) for the first time inthis work. Used modification resulted in increase of selectivity andsensitivity of the DNA voltammetric determination.
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