Lemna minor jako indikátor toxicity PAHs?
| Title in English | Lemna minor as the indicator of PAHs toxicity? |
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| Authors | |
| Year of publication | 2013 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Polycyclic aroamtic hydrocarbons represent an important group of organic contaminants in aquatic environment. Duckweed (Lemna minor L.), higher vascular plant, is commonly used for evaluation of xenobiotics phytotoxicity (US EPA, OECD). The aim of this study was to compare commonly used growth and physiological indicators of stressor effect with rarely studied biochemical and histochemical parameters of duckweed exposed to fluoranthene (FLT; 0.1 and 1 mg/L). After 4 and 10 days of FLT exposure only insignificant changes in biomass production, leaf area size, chlorophyll a,b and carotenoids content and maximal quantum yield of photosystem II (FV/FM) were found. In contrast to this, we proved an injury to cell membranes (increase of malon-dialdehyde content) and higher content of polyphenols due to considerably higher occurence of reactive oxygen species (hydrogen peroxide, superoxide) already in duckweed exposed to FLT 0.1 mg/L. Significant changes were found even in antioxidant mechanism activities (superoxide-dismutase, ascorbate-peroxidase). Standardized tests of organic pollutants phytotoxicity based just on growth parameters evaluation, do not irreversible changes in biochemical processes at the level of cells and tissues and thus the risk arising from the contaminated environment could be underestimated. |