On generating benchmark datasets for evaluation of segmentation and tracking algorithms in fluorescence microscopy
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| Year of publication | 2013 |
| MU Faculty or unit | |
| Citation | |
| Description | In fluorescence microscopy, the proper evaluation of image segmentation and tracking algorithms is still an open problem. As the ground truth for cell image data (and measurements on them) is not available in most experiments, the outputs of different image analysis methods can hardly be verified or compared to each other. We created a toolbox that can generate 3D digital phantoms of specific cellular components along with their corresponding images degraded by specific optics and electronics. The images can represent static scenes (fixed cell) as well as time-lapse sequences (living cells). Such synthetically generated images can serve as a benchmark dataset for measuring the quality of various segmentation and tracking algorithms. |
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