Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response

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Authors

KOLLAR Peter BÁRTA Tomáš HOŠEK Jan SOUČEK Karel MÜLLER ZÁVALOVÁ Veronika ARTINIAN Shushan TALHOUK Rabih ŠMEJKAL Karel SUCHÝ Pavel Jr. HAMPL Aleš

Year of publication 2013
Type Article in Periodical
Magazine / Source EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1155/2013/350519
Field Genetics and molecular biology
Keywords ROOT BARK; DIMETHYL-SULFOXIDE; IN-VITRO; KAPPA-B; CANCER; APOPTOSIS; DEATH; LINE; DIFFERENTIATION; PROGRESSION
Description Morus alba L. (MA) is a natural source of many compounds with different biological effects. It has been described to possess anti-inflammatory, antioxidant, and hepatoprotective activities. The aim of this study was to evaluate cytotoxicity of three flavonoids isolated from MA(kuwanon E, cudraflavone B, and 4'-O-methylkuwanon E) and to determine their effects on proliferation of THP-1 cells, and on cell cycle progression of cancer cells. Anti-inflammatory effects were also determined for all three given flavonoids. Methods used in the study included quantification of cells by hemocytometer and WST-1 assays, flow cytometry, western blotting, ELISA, and zymography. From the three compounds tested, cudraflavone B showed the strongest effects on cell cycle progression and viability of tumor and/or immortalized cells and also on inflammatory response of macrophage-like cells. Kuwanon E and 4'-O-methylkuwanon E exerted more sophisticated rather than direct toxic effect on used cell types. Our data indicate that mechanisms different from stress-related or apoptotic signaling pathways are involved in the action of these compounds. Although further studies are required to precisely define the mechanisms of MA flavonoid action in human cancer and macrophage-like cells, here we demonstrate their effects combining antiproliferative and anti-inflammatory activities, respectively.
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