Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Separation Science |
| MU Faculty or unit | |
| Citation | |
| Web | http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300926/abstract |
| Doi | http://dx.doi.org/10.1002/jssc.201300926 |
| Field | Biochemistry |
| Keywords | metabolomics; nucleotides; Paracoccus denitrificans; stem cells; system biology |
| Description | A new capillary zone electrophoretic method was developed for the determination of twelve purine and pyrimidine nucleotides, two adenine coenzymes and their reduced forms, and acetyl coenzyme A in various cell extracts. As the concentration levels of these metabolites in living cells are low; the capillary zone electrophoresis was combined with field-enhanced sample stacking. As a result, the separation conditions were optimised to achieve a suitable resolution at the relatively high sample volume provided by this on-line pre-concentration technique. |
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