Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis

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Authors

MUSILOVÁ Jindra KLEJDUS Bořivoj GLATZ Zdeněk

Year of publication 2013
Type Article in Periodical
Magazine / Source Journal of Separation Science
MU Faculty or unit

Faculty of Science

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300926/abstract
Doi http://dx.doi.org/10.1002/jssc.201300926
Field Biochemistry
Keywords metabolomics; nucleotides; Paracoccus denitrificans; stem cells; system biology
Description A new capillary zone electrophoretic method was developed for the determination of twelve purine and pyrimidine nucleotides, two adenine coenzymes and their reduced forms, and acetyl coenzyme A in various cell extracts. As the concentration levels of these metabolites in living cells are low; the capillary zone electrophoresis was combined with field-enhanced sample stacking. As a result, the separation conditions were optimised to achieve a suitable resolution at the relatively high sample volume provided by this on-line pre-concentration technique.
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