Determination of asymmetric and symmetric dimethylarginines in human plasma using high-performance liquid chromatography with electrochemical detection
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Separation Science |
| MU Faculty or unit | |
| Citation | |
| Web | http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300813/abstract |
| Doi | http://dx.doi.org/10.1002/jssc.201300813 |
| Field | Biochemistry |
| Keywords | L Arginine; Asymmetric dimethylarginine; Electrochemical detection; HPLC; Symmetric dimethylarginine |
| Attached files | |
| Description | A new HPLC method was developed and validated for the determination of asymmetric and symmetric dimethylarginines and L arginine in human plasma. After a SPE and evaporation of the eluate, the samples were derivatized with an o-phthaldialdehyde reagent containing 3 mercaptopropionic acid. The derivatives formed were analysed by an isocratic RP HPLC and electrochemical detection at +320 mV. |
| Related projects: |