Možnosti studia enzymových reakcí pomocí kapilární elektroforézy v on-line uspořádání

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Title in English Study of Enzymatic Reaction by On-line Capillary Electrophoresis.
Authors

ŘEMÍNEK Roman ZEISBERGEROVÁ Marta GLATZ Zdeněk

Year of publication 2013
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords metabolomics; metabolome; metabolism; NMR; MS; GS; HPLC; CE
Description This review provides overview of techniques used for merging of consecutively injected plugs of reactants within in-capillary enzymatic assays. The article is divided as follows: after an introduction, general features of on-line capillary electrophoresis and principles and applications of electrophoretically mediated microanalysis, at-inlet reactions and transverse diffusion of laminar flow profiles techniques are consecutively discussed and illustrated. Future directions are outlined in conclusion.
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