On-line CE/ESI/MS interfacing: Recent developments and applications in proteomics

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Authors

KRENKOVA Jana FORET František

Year of publication 2012
Type Article in Periodical
Magazine / Source Proteomics
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/pmic.201200140/pdf
Doi http://dx.doi.org/10.1002/pmic.201200140
Field Biochemistry
Keywords CE; Interfacing; Mass spectrometry Technology
Description Coupling of liquid-phase separation techniques with MS is the main way for obtaining identity and structural information in many fields of bioanalysis including proteomics. Although dominated by mature LC/MS technology there are also other techniques playing important roles in specific bioanalytical areas. CE offers different selectivity, higher efficiency, and often also shorter analysis time compared to HPLC. In addition, working with narrow, open separation capillaries and very small (nL) injection volumes may be an advantage when sample amount is limited or for a second dimension in multidimensional separations. Finally, once optimized, electrophoretic separation protocols can be easily Coupling ofCEwithMS as a powerful combination for performing rapid, efficient, and sensitive analysis has been confirmed by a significant number of original and review articles dealingwithCE/MS published since 2009. is discussed.
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