Development and validation of a general appoach to predict and quantify the synergism of anti-cancer drugs using experimental design and artificial neural networks

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Authors

PIVETTA Tiziana ISAIA Francesco TRUDU Federica PANI Alessandra MANCA Matteo PERRA Daniela AMATO Filippo HAVEL Josef

Year of publication 2013
Type Article in Periodical
Magazine / Source Talanta
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1016/j.talanta.2013.04.031
Field Analytic chemistry
Keywords synergism of drugs; artificial neural network; experimental design; cancer
Description The combination of two or more drugs using multidrug mixtures is a trend in the treatment of cancer. The goal is to search for a synergistic effect and thereby reduce the required dose and inhibit the development of resistance. An advanced model-free approach for data exploration and analysis, based on artificial neural networks (ANN) and experimental design is proposed to predict and quantify the synergism of drugs. The proposed method non-linearly correlates the concentrations of drugs with the cytotoxicity of the mixture, providing the possibility of choosing the optimal drug combination that gives the maximum synergism. The use of ANN allows for the prediction of the cytotoxicity of each combination of drugs in the chosen concentration interval. The method was validated by preparing and experimentally testing the combinations with the predicted highest synergistic effect. In all cases, the data predicted by the network were experimentally confirmed. The method was applied to several binary mixtures of cisplatin and [Cu(1,10-orthophenanthroline)2(H2O)](ClO4)2, Cu(1,10- orthophenanthroline)(H2O)2(ClO4)2 or [Cu(1,10- orthophenanthroline)2(imidazolidine-2-thione)](ClO4)2. The cytotoxicity of the two drugs, alone and in combination, was determined against human acute T-lymphoblastic leukemia cells (CCRF-CEM). For all systems, a synergistic effect was found for selected combinations.
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