Electrochemical Transducer for Oligonucleotide Biosensor Based on the Elimination and Adsorptive Transfer Techniques

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Authors

TRNKOVÁ Libuše JELEN František OSZOS Mehmet

Year of publication 2011
Type Chapter of a book
MU Faculty or unit

Faculty of Science

Citation
Description Electrochemical biosensors are usually based on redox reactions that consume or produce electrons. Such a device can be represented by an indication electrode, which integrates receptor transducer element providing elective quantitative analytical information; recorded signals are proportional to analyte concentrations. there are several types of electrochemical transducers, from which amperometric transducers are most used in biosensors due to their high sensitivity and selectivity. Excpet an indication electrode, the electrochemical system contains other two electrodes, that is, a reference electrode and an auxiliary electrode. Our approach in electrochemical oligonucleotide (ODN) transducer is built on the adsorptive stripping voltammetric (AdSV) technique in connection with elimination voltammetry with linear scan (EVLS). Generally, EVLS enables the elimination of selected partial voltammetric currents and the conservatio of the other one contributing to the increase of current sensitivity, the expansion of electrode potential range (potential window) and the separation of overlapped voltammetric signals. The basic idea of EVLS procedure lies in the different dependencies of various voltammetric current components on the scan rate. The elimination result can be achieved by a function obtained by linear combination of total voltammetric currents measured at different scan rates.
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