SEM and ESEM Observation of Stem Cells. Comparative Study of Human Embryonic Stem Cell Morphology.

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Authors

NEDĚLA Vilém TIHLAŘÍKOVÁ Eva HAMPL Aleš SEDLÁČKOVÁ Miroslava

Year of publication 2012
Type Article in Periodical
Magazine / Source Imaging & Microscopy
MU Faculty or unit

Faculty of Medicine

Citation
Field Microbiology, virology
Keywords Biological Samples; SEM; ESEM; Human Embryonic Stem Cells
Description Studying biological samples with scanning electron microscopy has specific requirements for their preparation. Sample drying is a particularly critical operation for objects such as cultured cells. The requirement for damaging drying step can be eliminated using environmental scanning electron microscopy. This study compares dried and wet samples of cultured human embryonic stem cells. It points to the advantages of both methods and to the complementarity of the information that they provide.
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