Metabolomics: nucleotide and coenzyme determination in cells by capillary electrophoresis

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Title in English Metabolomics: nucleotide and coenzyme determination in cells by capillary electrophoresi
Authors

MUSILOVÁ Jindra GLATZ Zdeněk

Year of publication 2012
Type Article in Proceedings
Conference 9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords capillary electrophoresis; metabolomics
Description Capillary electrophoresis (CE) is popular and suitable method for the metabolome analysis particularly due to high-efficient separations of a diverse range of chemical compounds, short analysis times and low sample volume requirements. New separations conditions for selective and rapid determination of 17 energetically important metabolites by CE were optimized. Applicability of this new method to real samples was demonstrated on bacterium P. denitrificans and stem cells extracts.
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