Glutation jako marker fotoxidativního stresu u rostlin vystavených různým intenzitám záření. In-vitro studie.
| Title in English | Glutathione as a marker of photooxidative stress in plants exposed to different intensities of light: In-vitro study. |
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| Authors | |
| Year of publication | 2011 |
| Type | Article in Proceedings |
| Conference | Rostlinné biotechnologie |
| MU Faculty or unit | |
| Citation | |
| Web | full_text_available_here |
| Field | Botany |
| Keywords | glutathione; Potinara; in-vitro; bioreactor RITA |
| Description | Micropropagation of plants in vitro is fast and effective way to obtain proliferation of new plant material. However, in vitro cultivated plants may suffer from high light stress when transfered from in vitro conditions. In our study, we focused on orchid plants Potinara hybr. cultivated in vitro and their sensitivity to short-term light stress. The plants were cultivated in temporary immersion system (TIS) RITA. This system enables complete or partial submersion of plants with liquid nutritive medium. In our experiment, plants were submerged 7 times during 24 hours for 5 minutes. Plants were cultivated one month before beginning of the experiment. We investigated the short-term effects of several different light intensities of photosynthetic active radiation (PAR) on the formation of the antioxidative substrate glutathion . Plants were cultivated under low light intensities of PAR (15 micromol. m-2. s-1). During short-term experiment, the plants were exposed to either 800 or 1400 micromol.m-2.s-1 of high light PAR. This exposition lasted for 1 hour. High light-exposed plant material was then subsequently harvested in 20 minute intervals for consequent glutathione analyzes. Concentration of glutathione reduced and oxidized form was measured in plant extract by HPLC. Glutathione thiols were labeled with monobromobimane. When low PAR exposition was used, glutathione concentration showed small increase after 20 minutes, then decrease after 40 minutes and subsequent rapid increase after 60 minutes of the high light exposition. The response of plants to the higher high light PAR (1400 micromol.m-2.s-1) exposition was that the glutathione concentration was much lower after the first 20 minutes and rapid increase of its concentration was measured after 40 and 60 minutes after the high light treatment. |
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