Analysis of metallothionein and glutathione in prostate cells as markers of oxidative stress
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | It has been repeatedly reported that prostate cancer cells are characteristic by altered ability to uptake and accumulate zinc(II) ions as compared to healthy prostate tissue. In fact, there is no evidence or records about prostate tumours with unchanged ability to accumulate zinc. In healthy and tumour prostate, intracellular zinc(II) is buffered by numerous proteins (metalloenzymes, nucleoproteins and metalloproteins, in particular metallothioneins). Metallothioneins (MTs) are ubiquitous low-molecular mass cysteine-rich proteins, playing a key role in maintaining zinc(II) homeostasis. They are involved in protection of cells against oxidative stress in association with reduced glutathione (GSH). The present study is focused on zinc(II) treatment effect on prostatic cell lines PC-3 (tumour) and PNT1A (non-tumour). Oxidative status of cells was monitored by evaluation of expression of metallothionein isoforms 1A and 2A at the mRNA and protein level, glutathione (oxidised and reduced) after exposition to zinc (II) treatment. PNT1A cells produced 4.2-fold less MT compared to PC3. PNT1A cells showed 4.8-fold increase trend; PC-3 did show significant trend in MTl and MT2 protein level with nearly ten-fold increase after 100 micromol zinc(II) treatment. In terms of redox state. PNT1A had a predominance of reduced GSH forms(GSH:GSSG ratio > 1), when exposed to zinc(II) compared to PC-3, where predominance of oxidised forms remains in all concentrations. When precise electrochemical methods and fluorescent microscopy are performed together, accurate information for metal fluxes, its buffering by thiols compounds and monitoring of redox state become a powerful tool for understanding the role of oxidative stress in carcinogenesis. |
| Related projects: |